Association between Serum Kalirin Levels and the KALRN gene rs9289231 Polymorphism in Early-Onset Coronary Artery Disease

  • Afsaneh Shafiei Department of Biochemistry, Payame Noor University of Tehran, Tehran, Iran.
  • Younes Pilehvar-Soltanahmadi Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Shayan Ziaee Tehran Heart Center, Tehran Unversity of Medical Sciences, Tehran, Iran.
  • Mohsen Mofarrah Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Nosratollah Zarghami Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Keywords: KALRN protein, human • Polymorphism, single nucleotide • Genotype • Coronary artery disease

Abstract

Background: Recently, rs9289231 genetic variations of kalirin (KALRN) have been introduced as potential genetic markers for coronary artery disease (CAD). However, the influence of KALRN single-nucleotide polymorphisms (SNPs) on serum kalirin levels has not been investigated in CAD patients so far. Thus, the present study aimed to survey whether SNP T > G (rs9289231) was associated with the risk of early-onset CAD and serum kalirin levels among the study subjects. Methods: The rs9289231 polymorphism of the KALRN was genotyped in 512 subjects (61.5% male, mean age = 46.3 ± 7.1 y), comprising 268 subjects with angiographically diagnosed CAD and 244 controls using an HRM assay. Also, the levels of serum kalirin were compared between 133 CAD subjects and 123 controls using a sandwich ELISA assay.  Results: The CAD subjects had more frequently GG genotypes than the controls. The odds ratio (OR) remained significant after adjustment for known CAD risk factors (OR = 4.13, 95% CI: 2.48–9.10; p value < 0.001). A significant difference was also observed in that the G allele was more frequent among the CAD subjects. The G allele at the rs9289231 polymorphism was associated with a higher risk of CAD (OR = 2.11, 95% CI: 1.27–2.59; p value = 0.001). The mean kalirin level of the CAD patients was higher than that of the controls (p value = 0.041). No significant correlation was seen in the different genotypes with serum kalirin levels.Conclusion: The KALRN rs9289231 T > G variant was considerably related with an increased risk of early-onset CAD.  High kalirin levels were found in young CAD patients compared to the control subjects, with the levels not affected by the different genotypes of rs9289231.
Published
2018-05-23
How to Cite
1.
Shafiei A, Pilehvar-Soltanahmadi Y, Ziaee S, Mofarrah M, Zarghami N. Association between Serum Kalirin Levels and the KALRN gene rs9289231 Polymorphism in Early-Onset Coronary Artery Disease. jthc. 13(2):58-4.