Human Amniotic Membrane Mesenchymal Stem Cells-Derived Conditioned Medium Adjusts Inflammatory And Myofibrotic Factors In Vivo

Abstract

Background: Numerous people are diagnosed with heart failure (HF) or die from it. One of the most effective HF treatments is hAMSCs-CM therapy. For the first time, we used an animal model of heart failure to perform an experiment to determine the hAMSCs-CM's method of action, focusing on TGF-β/Galactin-3, MCP1, BNP, and Ald.

Methods: Using the categories of Control, HF, Culture Medium, and Conditioned Medium (CM), 40 rats were divided into 4 groups. All rats other than the control received an injection of isoproterenol (ISO). Rats given CM received CM, and rats given culture medium received culture media. Then, an ELISA was used to measure the serum fibrotic factors.

Results: hAMSCs-CM treatment dramatically reduced all variables in HF rats when compared to HF and culture media, while increasing them significantly in controls. Conclusion: Because hAMSCs-CM modified inflammatory and fibrogenic cytokines such as TGF-β/Galactin-3, MCP1, BNP, and Ald in ISO-stimulated HF male rats, our findings partially addressed the gap in HF therapy.